Rieger M, Brückner M, Schäfer M, Müller-Auer S
Genotype GmbH, Wilhelmsfeld, Germany.
Yeast. 1997 Sep 15;13(11):1077-90. doi: 10.1002/(SICI)1097-0061(19970915)13:11<1077::AID-YEA152>3.0.CO;2-Y.
The nucleotide sequences of five major regions from chromosome VII of Saccharomyces cerevisiae have been determined and analysed. These regions represent 203 kilobases corresponding to approximately one-fifth of the complete yeast chromosome VII. Two fragments originate from the left arm of this chromosome. The first one of about 15.8 kb starts approximately 75 kb from the left telomere and is bordered by the SK18 chromosomal marker. The second fragment covers the 72.6 kb region between the chromosomal markers CYH2 and ALG2. On the right chromosomal arm three regions, a 70.6 kb region between the MSB2 and the KSS1 chromosomal markers and two smaller regions dominated by the KRE11 marker and another one in the vicinity of the SER2 marker were sequenced. We found a total of 114 open reading frames (ORFs), 13 of which were completely overlapping with larger ORFs running in the opposite direction. A total of 44 yeast genes, the physiological functions of which are known, could be precisely mapped on this chromosome. Of the remaining 57 ORFs, 26 shared sequence homologies with known genes, among which were 13 other S. cerevisiae genes and five genes from other organisms. No homology with any sequence in the databases could be found for 31 ORFs. Furthermore, five Ty elements were found, one of which may not be functional due to a frame shift in its Ty1B amino acid sequence. The five chromosomal regions harboured five potential ARS elements and one sigma element together with eight tRNA genes and two snRNAs, one of which is encoded by an intron of a protein-coding gene.
已对酿酒酵母七号染色体的五个主要区域的核苷酸序列进行了测定和分析。这些区域代表203千碱基，约占完整酵母七号染色体的五分之一。两个片段来自该染色体的左臂。第一个片段约15.8 kb，从左末端约75 kb处开始，以SK18染色体标记为边界。第二个片段覆盖CYH2和ALG2染色体标记之间的72.6 kb区域。在染色体右臂上，对三个区域进行了测序，分别是MSB2和KSS1染色体标记之间的70.6 kb区域，以及由KRE11标记主导的两个较小区域和SER2标记附近的另一个区域。我们总共发现了114个开放阅读框（ORF），其中13个与反向运行的较大ORF完全重叠。总共44个已知生理功能的酵母基因可以精确地定位在这条染色体上。在其余的57个ORF中，26个与已知基因具有序列同源性，其中包括13个其他酿酒酵母基因和5个来自其他生物体的基因。31个ORF在数据库中未发现与任何序列的同源性。此外，发现了五个Ty元件，其中一个由于其Ty1B氨基酸序列中的移码可能没有功能。这五个染色体区域共含有五个潜在的自主复制序列（ARS）元件和一个sigma元件，以及八个tRNA基因和两个snRNA，其中一个由蛋白质编码基因的内含子编码。
