Karim M M, Yamamoto M, Itoh H
Department of Ophthalmology, Kobe University School of Medicine.
Kobe J Med Sci. 1996 Jun;42(3):151-61.
The clinical features of three cases of retinoblastoma and the histopathological and immunocytochemical findings of the retinoblastoma specimens of these patients are described. The other eye of each patient was normal. There was no family history of retinoblastoma. Fundal examination disclosed white masses in the posterior segments of the eyes. B-scan ultrasonography showed echo dense areas in the masses. Orbital tomograms showed no evidence of tissue densities. There was neither extraocular extension nor optic nerve involvement. Immunocytochemistry of paraffin-embedded tissues of the neoplasms revealed immunoreactivity with antibodies against S-100 protein, glial fibrillary acidic protein(GFAP), vimentin, neuron-specific enolase(NSE) and bcl-2. S-100, GFAP and vimentin were positive only for glial elements(reactive astrocytes) and, NSE and bcl-2 for almost all tumor cells. These findings support the previous reports that the majority of retinoblastomas are sporadic. The results of this study also support the views that retinoblastomas are composed of neuron-committed cells.
本文描述了3例视网膜母细胞瘤患者的临床特征以及这些患者视网膜母细胞瘤标本的组织病理学和免疫细胞化学检查结果。每位患者的另一只眼均正常。无视网膜母细胞瘤家族史。眼底检查发现眼后段有白色肿物。B超显示肿物内回声密集区。眼眶断层扫描未显示组织密度证据。无眼外扩展及视神经受累。肿瘤石蜡包埋组织的免疫细胞化学显示，肿瘤对S-100蛋白、胶质纤维酸性蛋白（GFAP）、波形蛋白、神经元特异性烯醇化酶（NSE）和bcl-2抗体呈免疫反应性。S-100、GFAP和波形蛋白仅对神经胶质成分（反应性星形胶质细胞）呈阳性，而NSE和bcl-2对几乎所有肿瘤细胞呈阳性。这些发现支持了先前的报道，即大多数视网膜母细胞瘤是散发性的。本研究结果也支持视网膜母细胞瘤由神经定向细胞组成的观点。
