Terenghi G, Polak J M, Ballesta J, Cocchia D, Michetti F, Dahl D, Marangos P J, Garner A
Virchows Arch A Pathol Anat Histopathol. 1984;404(1):61-73. doi: 10.1007/BF00704251.
An immunocytochemical study of 30 retinoblastomas was carried out using antibodies to neuronal and glial markers. The tumours were found to react with antibodies to neuron-specific enolase (NSE), a marker for neuronal elements, and S-100 and glial fibrillary acidic protein (GFAP), both of which are proteins present in glia. Two distinct cell populations were found within the tumour: the first, composed of anaplastic tumour cells at various stages of differentiation, showed both NSE and S-100 immunoreactivity; the second cell type, which immuno-stained for S-100 and GFAP, resembled mature glial cells. The results of this study indicate that the retinoblastoma may arise from a pluripotential primitive cell partially retaining neuronal and glial characteristics.
利用针对神经元和神经胶质标志物的抗体，对30例视网膜母细胞瘤进行了免疫细胞化学研究。发现这些肿瘤与神经元特异性烯醇化酶（NSE，一种神经元成分的标志物）抗体以及S-100和胶质纤维酸性蛋白（GFAP，两者均为神经胶质中存在的蛋白质）抗体发生反应。在肿瘤内发现了两种不同的细胞群：第一种由处于不同分化阶段的间变性肿瘤细胞组成，显示出NSE和S-100免疫反应性；第二种细胞类型对S-100和GFAP进行免疫染色，类似于成熟的神经胶质细胞。这项研究结果表明，视网膜母细胞瘤可能起源于部分保留神经元和神经胶质特征的多能原始细胞。
