空肠弯曲菌的aroA基因。

The aroA gene of Campylobacter jejuni.

作者信息

Wösten M M, Dubbink H J, van der Zeijst B A

机构信息

Department of Bacteriology, Faculty of Veterinary Medicine, State University of Utrecht, The Netherlands.

出版信息

Gene. 1996 Nov 28;181(1-2):109-12. doi: 10.1016/s0378-1119(96)00482-9.

DOI:10.1016/s0378-1119(96)00482-9

PMID:8973316

Abstract

The gene for 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase (aroA) cloned from Campylobacter jejuni (Cj) strain 81116 was identified by complementation of an Escherichia coli (Ec) auxotrophic aroA mutant. The Cj aroA gene has been sequenced. It encodes an enzyme of 428 amino acids (aa), that is homologous to other bacterial EPSP synthases, especially that of Bacillus subtilis with which it has a 39% aa identity. The transcriptional start point was mapped. It is present in an upstream open reading frame (ORF) that has a strong homology to the gene encoding phenylalanine tRNA synthetase (pheS). Downstream from aroA another ORF is present which is homologous to the lytB gene of Ec. The stop codon of the aroA gene overlaps the start codon of lytB.

摘要

通过对大肠杆菌（Ec）营养缺陷型aroA突变体进行互补，鉴定出了从空肠弯曲菌（Cj）81116菌株克隆的5-烯醇丙酮酸莽草酸-3-磷酸（EPSP）合酶（aroA）基因。已对Cj aroA基因进行了测序。它编码一种由428个氨基酸（aa）组成的酶，与其他细菌的EPSP合酶同源，尤其是与枯草芽孢杆菌的EPSP合酶同源，二者氨基酸序列一致性为39%。已确定了转录起始点。它存在于一个上游开放阅读框（ORF）中，该开放阅读框与编码苯丙氨酸tRNA合成酶（pheS）的基因具有很强的同源性。aroA下游存在另一个ORF，它与Ec的lytB基因同源。aroA基因的终止密码子与lytB的起始密码子重叠。