百日咳博德特氏菌aroA基因的克隆及核苷酸序列
Cloning and nucleotide sequence of the aroA gene of Bordetella pertussis.
作者信息
Maskell D J, Morrissey P, Dougan G
机构信息
Department of Molecular Biology, Wellcome Research Laboratories, Beckenham, Kent, United Kingdom.
出版信息
J Bacteriol. 1988 Jun;170(6):2467-71. doi: 10.1128/jb.170.6.2467-2471.1988.
Abstract
The aroA locus of Bordetella pertussis, encoding 5-enolpyruvylshikimate 3-phosphate synthase, has been cloned into Escherichia coli by using a cosmid vector. The gene is expressed in E. coli and complemented an E. coli aroA mutant. The nucleotide sequence of the B. pertussis aroA gene was determined and contains an open reading frame encoding 442 amino acids, with a calculated molecular weight for 5-enolpyruvylshikimate 3-phosphate synthase of 46,688. The amino acid sequence derived from the nucleotide sequence shows homology with the published amino acid sequences of aroA gene products of other microorganisms.
摘要
百日咳博德特氏菌的aroA基因座编码5-烯醇丙酮酸莽草酸-3-磷酸合酶，已通过黏粒载体克隆到大肠杆菌中。该基因在大肠杆菌中表达，并使大肠杆菌aroA突变体得到互补。测定了百日咳博德特氏菌aroA基因的核苷酸序列，其包含一个编码442个氨基酸的开放阅读框，计算得出的5-烯醇丙酮酸莽草酸-3-磷酸合酶分子量为46,688。从核苷酸序列推导的氨基酸序列与其他微生物aroA基因产物的已发表氨基酸序列具有同源性。
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