Tsuboyama N, Hosokawa Y, Totani M, Oka J, Matsumoto A, Koide T, Kodama H
Department of Chemistry, Kochi Medical School, Japan.
Gene. 1996 Nov 28;181(1-2):161-5. doi: 10.1016/s0378-1119(96)00496-9.
Cysteine dioxygenase (CDO) is a key enzyme involved in the metabolism of L-cysteine. Genomic clones containing the 5'-flanking sequence of the rat CDO gene were isolated and characterized. The CDO gene spanned about 15 kb, and comprised 5 exons. All boundaries between the exons and introns matched the GT/AG rule. The major transcription start point (tsp) was A at 213 bp upstream from the ATG codon. The 5'-flanking region contained a TATA-box-like sequence and putative cis-acting regulatory elements. The 3' end of CDO was polyadenylated at several sites. Northern blots of RNA from rat tissues revealed the highest CDO mRNA level in the liver. Significant levels were observed in the kidney, lung and brain, implying tissue-specific differences in CDO promoter function.
半胱氨酸双加氧酶（CDO）是参与L-半胱氨酸代谢的关键酶。分离并鉴定了包含大鼠CDO基因5'侧翼序列的基因组克隆。CDO基因跨度约15kb，由5个外显子组成。外显子和内含子之间的所有边界均符合GT/AG规则。主要转录起始点（tsp）位于ATG密码子上游213bp处的A。5'侧翼区域包含一个类TATA盒序列和假定的顺式作用调节元件。CDO的3'末端在多个位点进行了多聚腺苷酸化。来自大鼠组织的RNA的Northern印迹显示肝脏中CDO mRNA水平最高。在肾脏、肺和脑中观察到显著水平，这意味着CDO启动子功能存在组织特异性差异。
