Martin B R, Dewey W L, Harris L S, Beckner J S
Res Commun Chem Pathol Pharmacol. 1977 Jul;17(3):457-70.
Pregnant dogs were administered intravenously 0.5 mg/kg of 3H-delta9-tetrahydrocannabinol and sacrificed at 30 minutes, the time of peak behavioral effects. The distribution of radioactivity in mothers and fetuses was quantified in many peripheral tissues and in major brain areas by sample combustion and liquid scintillation spectrometry. Brains were subfractionated by differential centrifugation in order to compare the subcellular localization of radioactivity in maternal and fetal brains. Although the level of radioactivity in maternal brain was three times higher than that in fetal brain, the distribution of radioactivity in the major brain areas was similar in mothers and fetuses. However, the subcellular distribution of radioactivity in whole brain homogenate of fetal dogs was markedly different from that in whole brain homogenates from mothers and appeared to be related directly to differing phospholipid content.
给怀孕的狗静脉注射0.5毫克/千克的3H-δ9-四氢大麻酚，并在30分钟时（行为效应达到峰值的时间）将其处死。通过样品燃烧和液体闪烁光谱法对母体和胎儿许多外周组织及主要脑区的放射性分布进行了定量。通过差速离心对大脑进行亚分级分离，以比较母体和胎儿大脑中放射性的亚细胞定位。尽管母体大脑中的放射性水平比胎儿大脑中的高两倍，但母体和胎儿主要脑区的放射性分布相似。然而，胎狗全脑匀浆中放射性的亚细胞分布与母体全脑匀浆中的明显不同，并且似乎与不同的磷脂含量直接相关。
