全反式维甲酸和佛波醇12-肉豆蔻酸酯13-乙酸酯对人肝癌细胞表面β-1,4-半乳糖基转移酶活性的影响
Effect of all-trans-retinoic acid and phorbol 12-myristate 13-acetate on the activity of human hepatocellular carcinoma cell-surface beta-1,4-galactosyltransferase.
作者信息
Zhou D, Jiang S, Shen Z, Gu J
机构信息
Gene Research Center, Shanghai Medical University, People's Republic of China.
出版信息
Biochem J. 1996 Dec 1;320 ( Pt 2)(Pt 2):623-5. doi: 10.1042/bj3200623.
A human hepatocellular carcinoma cell line, SMMC-7721, was treated with all-trans-retinoic acid (RA) and phorbol 12-myristate 13-acetate (PMA) to induce its differentiation and proliferation respectively. A biantennary sugar chain fluorescently labelled with 2-aminopyridine (PA), GlcNAc beta 1-2Man alpha 1-6(GlcNAc beta 1-2Man alpha 1-3)Man beta 1-4GlcNAc beta 1-4GlcNAc-PA, was used to detect the activity of beta-1,4-galactosyltransferase on the cell surface by HPLC. The results show that the activity of beta-1,4-galactosyltransferase on the cell surface increases when the cell is treated with RA, but decreases when it is treated with PMA, whereas the activities of the whole cell remain stable during the treatments.
用人全反式维甲酸（RA）和佛波酯（PMA）分别处理人肝癌细胞系SMMC-7721，以诱导其分化和增殖。用2-氨基吡啶（PA）荧光标记的双触角糖链GlcNAcβ1-2Manα1-6(GlcNAcβ1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc-PA，通过高效液相色谱法检测细胞表面β-1,4-半乳糖基转移酶的活性。结果表明，用RA处理细胞时，细胞表面β-1,4-半乳糖基转移酶的活性增加，而用PMA处理时活性降低，而在处理过程中全细胞的活性保持稳定。
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