Takumida M, Anniko M
Department of Otolaryngology, Hiroshima University School of Medicine, Japan.
Acta Otolaryngol. 1996 Nov;116(6):817-23. doi: 10.3109/00016489609137932.
The effect of gentamicin on the cytoskeletal organization of guniea pig vestibular sensory cells was investigated employing the saponin perfusion method and scanning electron microscopy. The intermediate filaments and microtubules were noted to have degenerated following gentamicin intoxication. Such degeneration was not linked with primary mitochondrial damage, but closely related to subsequent degeneration of Golgi apparatus and of endoplasmic reticulum. These findings suggest that microtubules and intermediate filaments work closely together to maintain the structural integrity of both the Golgi apparatus and the membrane-bound organelles, which can be altered by ototoxic drugs before the degeneration of membrane-bound organelles.
采用皂素灌注法和扫描电子显微镜研究庆大霉素对豚鼠前庭感觉细胞细胞骨架组织的影响。结果发现，庆大霉素中毒后中间丝和微管发生退化。这种退化与原发性线粒体损伤无关，但与随后高尔基体和内质网的退化密切相关。这些发现表明，微管和中间丝共同作用以维持高尔基体和膜结合细胞器的结构完整性，在膜结合细胞器退化之前，耳毒性药物可改变这种结构完整性。
