Takumida M, Miyawaki H, Harada Y
Department of Otolaryngology, Hiroshima University School of Medicine, Japan.
Auris Nasus Larynx. 1994;21(2):84-9. doi: 10.1016/s0385-8146(12)80025-3.
The cytoskeletal organization of the guinea pig vestibular sensory epithelial cells were investigated by the use of saponin perfusion method using scanning electron microscopy. The skeletal framework of a cell is composed of thin (actin or intermediate filaments) and thick filaments (microtubules). The membrane bound organelles such as nucleus, mitochondria, endoplasmic reticulum, Golgi apparatus, etc. were also well demonstrated. This made it possible to investigate the three-dimensional structures of cytoskeletons as well as their complex interactions with various membranes bound organelles. It is therefore suspected that this technique may provide us further information about distribution, topographic relationships, and the functional role of cytoskeletons.
利用皂素灌注法结合扫描电子显微镜对豚鼠前庭感觉上皮细胞的细胞骨架组织进行了研究。细胞的骨架框架由细（肌动蛋白或中间丝）和粗丝（微管）组成。细胞核、线粒体、内质网、高尔基体等膜结合细胞器也得到了很好的展示。这使得研究细胞骨架的三维结构以及它们与各种膜结合细胞器的复杂相互作用成为可能。因此，人们怀疑这项技术可能会为我们提供有关细胞骨架的分布、拓扑关系和功能作用的更多信息。
