Privezentsev K V, Sirota N P, Gaziev A I
Tsitol Genet. 1996 May-Jun;30(3):45-51.
Genetic effects of cadmium were studied using nucleoid microelectrophoresis of separate cells and micronucleus test. It was shown that cadmium chloride (in the range 2 x 10(-5) - 2 x 10(-1) mg of Cd2+/ml) induced nucleoid relaxation in peripheral blood lymphocytes (PBL) after one hour incubation in vitro. Single injection of cadmium chloride (1 mg of Cd2+/kg of body wt.) induced DNA damages in PBL, splenocytes and thymocytes. The dynamics of DNA damage and repair was distinct in different cells. The same dose induced an increase in the frequency of micronucleated bone marrow erythrocytes in mice. It is suggested that the mechanism of cadmium genotoxicity is due to the single strand breaks in DNA through the direct cadmium-DNA interactions as well as to the action of incision nucleases and/or DNA-glicosilases during DNA repair.
利用单细胞核仁微电泳和微核试验研究了镉的遗传效应。结果表明，在体外孵育一小时后，氯化镉（浓度范围为2×10⁻⁵ - 2×10⁻¹mg Cd²⁺/ml）可诱导外周血淋巴细胞（PBL）的核仁松弛。单次注射氯化镉（1mg Cd²⁺/kg体重）可诱导PBL、脾细胞和胸腺细胞中的DNA损伤。不同细胞中DNA损伤和修复的动态变化各不相同。相同剂量可导致小鼠骨髓微核红细胞频率增加。有人认为，镉的遗传毒性机制是由于镉与DNA直接相互作用导致DNA单链断裂，以及在DNA修复过程中内切核酸酶和/或DNA糖基化酶的作用。
