Latinwo L M, Ikediobi C O, Singh N P, Sponholtz G, Fasanya C, Riley L
Department of Biology, Florida A and M University, Tallahassee, 32307, USA.
Cell Mol Biol (Noisy-le-grand). 1997 Mar;43(2):203-10.
Cadmium chloride-induced DNA damage was investigated in individual brain, kidney and liver cells isolated from rats gavaged 14 mg/kg/day cadmium chloride. Animals were sacrificed on days 2, 4, 8, 16, and 33, and DNA damage was determined using the recently developed alkaline microgel electrophoresis technique. Data for DNA migration from 50 randomly selected cells clearly show significant increases in DNA damage in cells from three different organs of cadmium chloride gavaged animals compared to saline treated control animals (33 day control, brain 64.7 +/- 5.3, kidney 75.5 +/- 9.4, liver 67.9 +/- 5.7 microm; 33 days experimental, brain 284.3 +/- 16.9, kidney 397.9 +/- 11.3, liver 315 +/- 22.5 microm; these values represent length of exposure in days and length of DNA migration in micron). There was an increase in DNA damage for all three cell types, with increasing duration of treatment. Cadmium (CdCl2) induced levels of DNA single strand breaks were more pronounced in kidney cells than in cells from the other two organs. Body and organ weights decreased of treated animals were decreased as compared to control. Results of this study indicate a potential of cadmium to be a genotoxic compound.
研究了给大鼠灌胃14毫克/千克/天的氯化镉后，从其分离出的单个脑细胞、肾细胞和肝细胞中氯化镉诱导的DNA损伤情况。在第2、4、8、16和33天处死动物，并使用最近开发的碱性微凝胶电泳技术测定DNA损伤。从50个随机选择的细胞中获得的DNA迁移数据清楚地表明，与生理盐水处理的对照动物相比，灌胃氯化镉的动物的三种不同器官的细胞中DNA损伤显著增加（33天对照组，脑64.7±5.3，肾75.5±9.4，肝67.9±5.7微米；33天实验组，脑284.3±16.9，肾397.9±11.3，肝315±22.5微米；这些值分别代表暴露天数和DNA迁移长度，单位为微米）。随着处理时间的延长，所有三种细胞类型的DNA损伤都有所增加。镉（CdCl2）诱导的DNA单链断裂水平在肾细胞中比在其他两个器官的细胞中更明显。与对照组相比，处理动物的体重和器官重量均下降。本研究结果表明镉有可能是一种遗传毒性化合物。
