鱼精蛋白作用导致的单核细胞增生李斯特菌和腐败希瓦氏菌细胞形态变化。
Changes in cell morphology of Listeria monocytogenes and Shewanella putrefaciens resulting from the action of protamine.
作者信息
Johansen C, Gill T, Gram L
机构信息
Ministry of Agriculture and Fisheries, Danish Institute for Fisheries Research, Technical University of Denmark, Lyngby.
出版信息
Appl Environ Microbiol. 1996 Mar;62(3):1058-64. doi: 10.1128/aem.62.3.1058-1064.1996.
Abstract
Protamine, which is an antibacterial basic peptide, was shown to alter the cell morphology of Listeria monocytogenes and Shewanella putrefaciens. Atomic force microscopy revealed that protamine smoothed the surface of cells, formed holes in the cell envelope, and caused fusion of S. putrefaciens cells. Immunoelectron microscopy of protamine-treated cells of both L. monocytogenes and S. putrefaciens showed great damage to the cell wall and condensation of the cytoplasm. Respiration of the cells was decreased due to treatment with sublethal concentrations of protamine, probably due to leakage or loss of cell envelope potential. It was concluded that protamine disrupted the outer surface structure and condensed the cytoplasm of sensitive cells and, in sublethal concentrations, altered membrane structures, thereby eliminating respiration.
摘要
鱼精蛋白是一种抗菌碱性肽，已被证明能改变单核细胞增生李斯特菌和腐败希瓦氏菌的细胞形态。原子力显微镜显示，鱼精蛋白使细胞表面变得光滑，在细胞壁上形成孔洞，并导致腐败希瓦氏菌细胞融合。对经鱼精蛋白处理的单核细胞增生李斯特菌和腐败希瓦氏菌细胞进行免疫电子显微镜观察，结果显示细胞壁受到严重损伤，细胞质发生凝聚。用亚致死浓度的鱼精蛋白处理后，细胞呼吸减弱，这可能是由于细胞膜电位泄漏或丧失所致。得出的结论是，鱼精蛋白破坏了敏感细胞的外表面结构，使细胞质凝聚，在亚致死浓度下改变了膜结构，从而抑制了呼吸作用。
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