从类产碱假单胞菌F-111中纯化碱性脂肪酶并进行部分特性鉴定。
Purification and partial characterization of an alkaline lipase from Pseudomonas pseudoalcaligenes F-111.
作者信息
Lin S F, Chiou C M, Yeh C M, Tsai Y C
机构信息
Department of Bioengineering, Tatung Institute of Technology, Taipei, Taiwan, Republic of China.
出版信息
Appl Environ Microbiol. 1996 Mar;62(3):1093-5. doi: 10.1128/aem.62.3.1093-1095.1996.
Abstract
An extracellular alkaline lipase of alkalophilic Pseudomonas pseudoalcaligenes F-111 was purified to homogeneity. The apparent molecular weight determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis was 32,000, and the isoelectric point was 7.3. With p-nitrophenyl esters as its substrates, the enzyme shows preference for C12 acyl and C14 acyl groups. It was stable in the pH range of 6 to 10, which coincides with the optimum pH range.
摘要
嗜碱假产碱假单胞菌F-111的一种胞外碱性脂肪酶被纯化至同质。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定的表观分子量为32,000，等电点为7.3。以对硝基苯酯为底物时，该酶对C12酰基和C14酰基表现出偏好。它在pH 6至10的范围内稳定，这与最适pH范围一致。
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