Watanabe H, Mochizuki T, Maeyama K
Dept. of Pharmacology, Ehime University School of Medicine, Japan.
Scand J Gastroenterol. 1996 Dec;31(12):1144-50. doi: 10.3109/00365529609036902.
Since few studies of (penta)gastrin-induced histamine release from the gastric mucosa into blood has been performed, an effect of pentagastrin on histamine level of rat blood was examined by using the in vivo microdialysis method.
Pentagastrin was perfused through the microdialysis probe implanted into the jugular vein of urethane-anesthetized rats or in urethane-anesthetized, totally gastrectomized rats, and dialysis samples of blood were concurrently collected. Histidine decarboxylase (HDC) activities and histamine contents in the glandular stomach and gastric acid output after pentagastrin stimulation were also investigated.
Pentagastrin induced a transient increase of blood histamine in a dose-dependent manner but failed to cause any increase of blood histamine in the totally gastrectomized rat. Pentagastrin also induced increases of the HDC activity in the glandular stomach and of the gastric acid output. The peak histamine level in blood occurred 40 min after pentagastrin perfusion, whereas the peak acid secretion occurred after 80-120 min and then leveled off.
The transient increase of blood histamine induced by pentagastrin is attributable to the histamine released from enterochromaffin-like cells and could be monitored by using the in vivo microdialysis method.
由于关于（五肽）胃泌素诱导胃黏膜组胺释放进入血液的研究较少，因此采用体内微透析法研究了五肽胃泌素对大鼠血液组胺水平的影响。
将五肽胃泌素灌注到植入乌拉坦麻醉大鼠颈静脉的微透析探针中，或灌注到乌拉坦麻醉的全胃切除大鼠中，同时收集血液透析样本。还研究了五肽胃泌素刺激后腺胃中的组氨酸脱羧酶（HDC）活性、组胺含量和胃酸分泌量。
五肽胃泌素以剂量依赖性方式诱导血液组胺短暂升高，但在全胃切除大鼠中未能引起血液组胺升高。五肽胃泌素还诱导腺胃中HDC活性增加和胃酸分泌量增加。五肽胃泌素灌注后40分钟血液中组胺水平达到峰值，而胃酸分泌峰值出现在80 - 120分钟后，然后趋于平稳。
五肽胃泌素诱导的血液组胺短暂升高归因于肠嗜铬样细胞释放的组胺，并且可以通过体内微透析法进行监测。
