Barreto-Bergter E, Branquinha M H, Pohlentz G, Vermelho A B
Departamento de Microbiologia Geral, Universidade Federal do Rio de Janeiro, Brazil
J Eukaryot Microbiol. 1996 Nov-Dec;43(6):486-8.
A detailed knowledge of the primary structure of neutral glycosphingolipids isolated and purified from Trypanosoma dionisii has been elucidated using a combination of techniques--as column chromatography, HPTLC and GC-MS together with fast atom bombardment spectrometry. The ceramide monohexoside fraction (CMH) contained both glucosyl- and galactosylceramides in a ratio of 1:1, sphingosine and as fatty acyl groups mainly C-24 saturated and 2-hydroxy fatty acids. A close similarity between Trypanosoma cruzi and T. dionisii monohexosylceramides was reported.
通过柱色谱、高效薄层层析、气相色谱 - 质谱联用以及快原子轰击质谱等技术的组合，已阐明了从狄氏锥虫中分离和纯化的中性糖鞘脂一级结构的详细信息。神经酰胺单己糖苷部分（CMH）含有葡萄糖基神经酰胺和半乳糖基神经酰胺，比例为1:1，鞘氨醇以及作为脂肪酰基的主要是C - 24饱和脂肪酸和2 - 羟基脂肪酸。据报道，克氏锥虫和狄氏锥虫的单己糖神经酰胺之间有密切相似性。
