克氏锥虫中性糖鞘脂的结构表征

Structural characterization of neutral glycosphingolipids from Trypanosoma cruzi.

作者信息

Barreto-Bergter E, Vermelho A B, Hartmann R, Pohlentz G, Klein R A, Egge H

机构信息

Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Brazil.

出版信息

Mol Biochem Parasitol. 1992 Apr;51(2):263-70. doi: 10.1016/0166-6851(92)90076-v.

DOI:10.1016/0166-6851(92)90076-v

PMID:1574084

Abstract

The major neutral glycosphingolipids from Trypanosoma cruzi ceramide mono- and dihexosides (CMH and CDH, respectively) were analysed after chromatographic purification using 1H 500 MHz nuclear magnetic resonance spectroscopy and fast atom bombardment mass spectrometry. The ceramide monohexoside fraction (CMH) contained both glucosyl- and galactosylceramides. After peracetylation, the CMH fraction was separated into 2 subfractions, CMH-COH and CMH-Cn, containing either hydroxy fatty acids or n-fatty acids. In the CMH-COH fraction glucose and galactose were present in a ratio of 2:1, whereas this ratio was 1:1 in the CMH-Cn fraction. The CDH fraction was identified as lactosylceramide with sphingosine as the long chain base and 16:0, 18:0, and 24:0, 24:4 fatty acids as major components.

摘要

利用500兆赫的1H核磁共振光谱和快原子轰击质谱法，对经色谱纯化后的克氏锥虫主要中性糖鞘脂类物质（分别为神经酰胺单己糖苷和二己糖苷，即CMH和CDH）进行了分析。神经酰胺单己糖苷部分（CMH）包含葡糖基神经酰胺和半乳糖基神经酰胺。全乙酰化后，CMH部分被分离成两个亚组分，即CMH - COH和CMH - Cn，分别含有羟基脂肪酸或n - 脂肪酸。在CMH - COH部分中，葡萄糖和半乳糖的比例为2:1，而在CMH - Cn部分中该比例为1:1。CDH部分被鉴定为乳糖基神经酰胺，其长链碱基为鞘氨醇，主要成分脂肪酸为16:0、18:0以及24:0、24:4。