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无晶状体兔眼晶状体上皮细胞中α平滑肌肌动蛋白的存在。

Presence of alpha smooth muscle actin in lens epithelial cells of aphakic rabbit eyes.

作者信息

Kurosaka D, Kato K, Nagamoto T

机构信息

Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan.

出版信息

Br J Ophthalmol. 1996 Oct;80(10):906-10. doi: 10.1136/bjo.80.10.906.

DOI:10.1136/bjo.80.10.906
PMID:8976703
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC505647/
Abstract

AIMS

To determine whether alpha smooth muscle actin (alpha-SMA), a marker for myofibroblastic cells, is present in lens epithelial cells (LECs) in rabbit aphakic eyes.

METHODS

Phacoemulsification was performed in rabbit eyes, which were enucleated after surgery. Immunohistochemical methods were used to detect alpha-SMA in LECs.

RESULTS

Five days after surgery, the presence of alpha-SMA positive LECs was observed mainly around the adhesive portion of the anterior capsule margin and the posterior capsule. The posterior capsule was wrinkled at the adhesive portion. The alpha-SMA positive LECs were flattened with spindle-shaped cross sections. Seven days after surgery, the alpha-SMA positive LECs covered most of the central posterior capsule. They disappeared 10 days after surgery. On the other hand, the cuboidal LECs in the capsular bag were negative for alpha-SMA.

CONCLUSION

The flattened LECs with spindle-shaped cross sections observed 5 days after cataract surgery contained alpha-SMA. Such LECs were distinguished biochemically from the cuboidal LECs, which lacked alpha-SMA.

摘要

目的

确定肌成纤维细胞标志物α平滑肌肌动蛋白（α-SMA）是否存在于兔无晶状体眼的晶状体上皮细胞（LECs）中。

方法

对兔眼进行超声乳化手术，术后摘除眼球。采用免疫组织化学方法检测LECs中的α-SMA。

结果

术后5天，观察到α-SMA阳性的LECs主要出现在前囊边缘与后囊的粘连部位周围。后囊在粘连部位出现皱缩。α-SMA阳性的LECs呈扁平状，横切面呈纺锤形。术后7天，α-SMA阳性的LECs覆盖了大部分中央后囊。术后10天它们消失。另一方面，囊袋内的立方形LECs对α-SMA呈阴性。

结论

白内障手术后5天观察到的横切面呈纺锤形的扁平LECs含有α-SMA。此类LECs在生化特性上与缺乏α-SMA的立方形LECs不同。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9478/505647/1b00f7e9ef4b/brjopthal00010-0055-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9478/505647/414988dd8784/brjopthal00010-0053-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9478/505647/fb0118c069bb/brjopthal00010-0053-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9478/505647/1c0bfe6ab0a0/brjopthal00010-0054-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9478/505647/e72109f4f9c9/brjopthal00010-0054-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9478/505647/ea6f5fee1e8d/brjopthal00010-0055-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9478/505647/1b00f7e9ef4b/brjopthal00010-0055-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9478/505647/414988dd8784/brjopthal00010-0053-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9478/505647/fb0118c069bb/brjopthal00010-0053-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9478/505647/1c0bfe6ab0a0/brjopthal00010-0054-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9478/505647/e72109f4f9c9/brjopthal00010-0054-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9478/505647/ea6f5fee1e8d/brjopthal00010-0055-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9478/505647/1b00f7e9ef4b/brjopthal00010-0055-b.jpg

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