Sakuma S, Saya H, Tada M, Nakao M, Fujiwara T, Roth J A, Sawamura Y, Shinohe Y, Abe H
Department of Neurosurgery, University of Hokkaido School of Medicine, Kita-ku, Sapporo, Japan.
FEBS Lett. 1996 Dec 2;398(2-3):165-9. doi: 10.1016/s0014-5793(96)01234-3.
We have previously reported on radiation-induction of ptk-3 in rat astrocyte culture [Sakuma et al. (1995) Radiat. Res. 143, 1-7]. Ptk-3 was considered to be a rat version of human DDR (discoidin domain receptor). We cloned and analyzed genomic DNA of the DDR and its promoter region. We discovered that the promoter region contained a consensus sequence of the p53 tumor suppressor binding site. Adenovirus-mediated p53 transfection induced a high level of DDR mRNA in SAOS2 human osteosarcoma cells. These results indicate that DDR is up-regulated by the p53 protein.
我们之前曾报道过在大鼠星形胶质细胞培养中辐射诱导ptk - 3的情况[Sakuma等人（1995年），《辐射研究》143卷，第1 - 7页]。Ptk - 3被认为是人类盘状结构域受体（DDR）的大鼠版本。我们克隆并分析了DDR的基因组DNA及其启动子区域。我们发现启动子区域包含p53肿瘤抑制因子结合位点的共有序列。腺病毒介导的p53转染在SAOS2人骨肉瘤细胞中诱导了高水平的DDR mRNA。这些结果表明DDR由p53蛋白上调。
