Pádua M, Aubert S, Casimiro A, Bligny R
Laboratoire de Physiologie Cellulaire Végétale, URA CNRS No. 576, DBMS, CEA-Grenoble, France.
FEBS Lett. 1996 Dec 2;398(2-3):248-52. doi: 10.1016/s0014-5793(96)01260-4.
Sycamore suspension cells (Acer pseudoplatanus L.) were grown in the presence of sublethal concentrations of copper (50 microM). During the first 5-6 days of treatment, growth was not affected, but cell respiration (coupled and uncoupled) declined to approximately 60% of its normal value. This decline of respiration was attributed to a progressive diminution of the number of mitochondria in copper-treated cells, based on the demonstration of the concomitant decline of (1) cardiolipin (diphosphatidylglycerol) and cytochrome aa3 (cytochrome oxidase), two specific markers of mitochondrial inner membrane, and (2) fumarase activity, a specific marker of mitochondrial matrix space. In addition, the mitochondria extracted from copper-treated cells presented the same properties as those from control cells, concerning substrate oxidation, cardiolipin and cytochrome aa3 contents, and fumarase activity. These results strongly suggest that copper triggered an arrest of mitochondrial biogenesis, which preceded cell division arrest.
梧桐悬浮细胞（槭树）在亚致死浓度的铜（50微摩尔）存在下生长。在处理的前5 - 6天，生长未受影响，但细胞呼吸（偶联和非偶联）下降至其正常值的约60%。这种呼吸下降归因于铜处理细胞中线粒体数量的逐渐减少，这基于以下两点的同时下降得以证明：（1）心磷脂（二磷脂酰甘油）和细胞色素aa3（细胞色素氧化酶），这两种线粒体内膜的特异性标志物；（2）延胡索酸酶活性，线粒体基质空间的特异性标志物。此外，从铜处理细胞中提取的线粒体在底物氧化、心磷脂和细胞色素aa3含量以及延胡索酸酶活性方面呈现出与对照细胞相同的特性。这些结果有力地表明，铜引发了线粒体生物发生的停滞，这先于细胞分裂的停滞。
