Puri K D, Finger E B, Springer T A
Department of Pathology, Center for Blood Research, Harvard Medical School, Boston, MA 02115, USA.
J Immunol. 1997 Jan 1;158(1):405-13.
Selectins are a family of lectins that mediate tethering and rolling of leukocytes on endothelium in vascular shear flow. To test the hypothesis that the kinetics and the strength of rolling interactions can be independently varied for different selectin:ligand pairs, we have directly compared all three selectins with regard to distinct measures of selectin-mediated interactions in shear flow: tethering, rolling velocity, and strength of rolling adhesions. At comparable site densities of E-selectin, P-selectin, and the L-selectin counter-receptor CD34, neutrophils tethered with similar efficiency and developed rolling adhesions of similar strength as measured by resistance to detachment. Under the same conditions, neutrophils rolled 7.5- to 10.5-fold faster on CD34 than on E-selectin and P-selectin. These findings suggest that the kinetics of bond dissociation and bond formation are faster for L-selectin than for E- and P-selectin. We also compared the behavior of neutrophils and lymphocytes on the same selectin. Both cell types showed comparable strength of binding to CD34; however, neutrophils rolled with faster velocities than lymphocytes.
选择素是一类凝集素，可介导白细胞在血管剪切流中在内皮细胞上的拴系和滚动。为了验证不同选择素：配体对的滚动相互作用的动力学和强度可独立变化这一假设，我们直接比较了所有三种选择素在剪切流中选择素介导的相互作用的不同指标：拴系、滚动速度和滚动黏附强度。在E选择素、P选择素和L选择素的反受体CD34具有可比位点密度的情况下，中性粒细胞以相似的效率拴系，并形成了强度相似的滚动黏附，这通过抗脱离能力来衡量。在相同条件下，中性粒细胞在CD34上滚动的速度比在E选择素和P选择素上快7.5至10.5倍。这些发现表明，L选择素的键解离和键形成动力学比E选择素和P选择素更快。我们还比较了中性粒细胞和淋巴细胞在同一选择素上的行为。两种细胞类型与CD34结合的强度相当；然而，中性粒细胞滚动的速度比淋巴细胞快。
