Kühnlein P, Mitnacht R, Torres-Nagel N E, Herrmann T, Elbe A, Hünig T
Institute for Virology and Immunobiology, University of Würzburg, Germany.
Eur J Immunol. 1996 Dec;26(12):3092-7. doi: 10.1002/eji.1830261240.
Two monoclonal antibodies with specificity for rat gammadelta T cell receptor (TCR) were generated. One, called V65, reacts with all CD3+ alphabeta TCR- rat Tcells and thus recognizes a constant determinant of the rat gammadelta TCR (Kühnlein et al., Journal of Immunology 1994, 153: 979). The other, called V45, reacts with approximately 80% of gammadelta T cells in peripheral lymphoid organs. In rat epidermis, V65 but not V45 detects a dense network of the dendritic epidermal Tcells (DETC). Analysis of epidermal RNA by polymerase chain reaction (PCR) indicated that Vgamma3 and Vdelta1 are the predominant, if not exclusive TCR V transcripts present at this site. Sequence analysis of cDNA clones obtained by reverse transcription-PCR with Vgamma3- and Vdelta1-specific primers revealed that the variable domains of rat DETC gamma and delta chains are very homologous to those described in mice (92% and 95% identity at the protein level). The complete conservation between the two species of the amino acid sequences at the V-(D)-J transitions of this monomorphic receptor indicates that the interaction of the DETC TCR with its as yet unknown ligand must be of central importance for DETC function.
制备了两种对大鼠γδ T细胞受体（TCR）具有特异性的单克隆抗体。一种名为V65，可与所有CD3⁺αβ TCR⁻大鼠T细胞发生反应，因此识别大鼠γδ TCR的一个恒定决定簇（库恩莱因等人，《免疫学杂志》1994年，153: 979）。另一种名为V45，可与外周淋巴器官中约80%的γδ T细胞发生反应。在大鼠表皮中，V65而非V45可检测到树突状表皮T细胞（DETC）的密集网络。通过聚合酶链反应（PCR）对表皮RNA进行分析表明，Vγ3和Vδ1是该部位主要的（即便不是唯一的）TCR V转录本。用Vγ3和Vδ1特异性引物通过逆转录PCR获得的cDNA克隆的序列分析显示，大鼠DETC γ和δ链的可变区与小鼠中描述的可变区非常同源（蛋白质水平上的同一性分别为92%和95%）。在这种单态性受体的V-(D)-J连接处，两种物种氨基酸序列的完全保守表明，DETC TCR与其尚未明确的配体之间的相互作用对于DETC功能必定至关重要。
