Seppä H, Väänänen K, Korhonen K
Acta Histochem. 1979;64(1):64-70. doi: 10.1016/S0065-1281(79)80053-7.
The effects of purified mast cell chymase of rat skin on intercellular matrix were studied histochemically using cartilage as a model. The enzyme effectively releases proteoglycans from neonatal human costal cartilage. An analysis of the primary site of proteolytic attack by chymase in the matrix was attempted. The results suggest that components containing the more acidic radicals (e.g. sulphates) are hydrolyzed first. After a prolonged attack, the matrix loses most of its staining with Toluidine Blue and Alcian Blue, and staining of collagen is increased. The hydrolyzed regions of cartilage in Alcian Blue-stained sections exhibited a purple interference colour in polarized light. A similar interference colour appeared after incubating sections of rat skin with chymase. The mast cell chymase presumably promotes diffusion of plasma to the site of injury by the direct breakdown of connective tissue ground substance.
以软骨为模型，采用组织化学方法研究了大鼠皮肤纯化肥大细胞糜蛋白酶对细胞间基质的作用。该酶可有效从新生儿人肋软骨中释放蛋白聚糖。尝试分析糜蛋白酶在基质中蛋白水解攻击的主要位点。结果表明，含有更多酸性基团（如硫酸盐）的成分首先被水解。长时间攻击后，基质失去大部分甲苯胺蓝和阿尔辛蓝染色，而胶原蛋白染色增加。阿尔辛蓝染色切片中软骨的水解区域在偏振光下呈现紫色干涉色。用糜蛋白酶孵育大鼠皮肤切片后也出现了类似的干涉色。肥大细胞糜蛋白酶可能通过直接分解结缔组织基质促进血浆扩散到损伤部位。
