Averboukh L, Liang P, Kantoff P W, Pardee A B
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, USA.
Prostate. 1996 Dec;29(6):350-5. doi: 10.1002/(SICI)1097-0045(199612)29:6<350::AID-PROS2>3.0.CO;2-C.
The growth and function the normal prostate is dependent on the presence of androgen. As prostate tumors progress there is a loss of androgen-dependent cell growth. The identification of the genes that are regulated by androgens may be of pathological and clinical significance.
In this study the differential display method was used to identify genes regulated by androgen in an androgen-responsive prostate cancer cell line, LNCaP-FGC.
A gene whose expression is down-regulated in LNCaP-FGC cells after 30 hr of androgen deprivation has been identified. This gene is a previously identified member of the S100 gene family of calcium-binding proteins, namely S100P. Here we show that S100P expression is regulated by the synthetic androgen R1881, but not by serum growth factors. It is dysregulated in the androgen-independent prostate cancer cell lines LNCaP-R, DU145, and PC3.
The data indicate that S100P may play a role in the etiology of prostate cancer.
正常前列腺的生长和功能依赖于雄激素的存在。随着前列腺肿瘤的进展，雄激素依赖性细胞生长会丧失。鉴定受雄激素调控的基因可能具有病理学和临床意义。
在本研究中，采用差异显示法来鉴定雄激素应答性前列腺癌细胞系LNCaP-FGC中受雄激素调控的基因。
已鉴定出一个基因，其在雄激素剥夺30小时后在LNCaP-FGC细胞中的表达下调。该基因是先前已鉴定的钙结合蛋白S100基因家族的成员，即S100P。在此我们表明，S100P的表达受合成雄激素R1881调控，但不受血清生长因子调控。它在雄激素非依赖性前列腺癌细胞系LNCaP-R、DU145和PC3中表达失调。
数据表明S100P可能在前列腺癌的病因学中起作用。
