Lombardo M E, Meyer-Siegler K, Hakky S I, Hudson P B
Department of Veterans Affairs Medical Center, Bay Pines Florida 33504, USA.
Prostate. 1996 Dec;29(6):381-5. doi: 10.1002/(SICI)1097-0045(199612)29:6<381::AID-PROS6>3.0.CO;2-B.
The increasing incidence of prostate cancer demands that we give our full attention not only to the etiology and prevention of this common type of cancer, but also to the diagnosis and prognostic course of this disease. In an effort to develop new prostatic tumor markers that could be useful to the physician at the current state of our knowledge in the diagnosis and prognosis of this disease, our laboratories have undertaken an effort to isolate and characterize the nature of the major proteins in the normal prostate and in prostatic neoplasia.
In this preliminary study, tissue was obtained from open prostatic surgery in patients with a pre- and postoperative diagnosis of benign prostatic hyperplasia. The initial fractionation and separation of the proteins was achieved through the use of ultrafiltration of homogenates followed by SDS-PAGE. Initial analysis of four prominent protein bands was accomplished by amino acid sequencing, and identified by a search in GeneBank data base.
Two proteins previously identified in prostatic tissue were prostate specific antigen (M(r) 26,496) with 240 amino acid residues and beta-inhibin (M(r) 10,704) with 94-amino acid residues. A third protein was identified as human cysteine rich protein (hCRP). This protein functions as a DNA binding protein and has previously been postulated to contain four putative zinc fingers and to play a fundamental role in cellular function. Ubiquitin, the fourth major protein identified was a 76-amino acid polypeptide whose function is to target other proteins for destruction.
hCRP and ubiquitin are reported as being found in high levels in prostatic tissue for the first time.
前列腺癌发病率的不断上升要求我们不仅要充分关注这种常见癌症的病因和预防，还要关注该疾病的诊断和预后过程。为了开发新的前列腺肿瘤标志物，以便在我们目前对该疾病诊断和预后的认知水平下为医生提供帮助，我们的实验室致力于分离和鉴定正常前列腺组织及前列腺肿瘤中主要蛋白质的性质。
在这项初步研究中，从术前和术后诊断为良性前列腺增生的患者开放性前列腺手术中获取组织。通过对匀浆进行超滤，随后进行SDS - PAGE，实现蛋白质的初步分级分离。通过氨基酸测序对四条突出的蛋白带进行初步分析，并在基因库数据库中进行搜索鉴定。
先前在前列腺组织中鉴定出的两种蛋白质分别是前列腺特异性抗原（分子量26,496），含240个氨基酸残基，以及β - 抑制素（分子量10,704），含94个氨基酸残基。第三种蛋白质被鉴定为人富含半胱氨酸蛋白（hCRP）。这种蛋白质作为一种DNA结合蛋白发挥作用，先前推测它含有四个假定的锌指结构，并在细胞功能中起重要作用。鉴定出的第四种主要蛋白质泛素是一种76个氨基酸的多肽，其功能是将其他蛋白质靶向降解。
首次报道hCRP和泛素在前列腺组织中含量较高。
