Kaufmann R, Lindschau C, Höer A, Henklein P, Adomeit A, Haller H, Liebmann C, Oberdisse E, Nowak G
Max Planck Gesellschaft, Research Unit, Pharmacological Hemostaseology, Friedrich Schiller University Jena, Germany.
J Neurosci Res. 1996 Dec 15;46(6):641-51. doi: 10.1002/(SICI)1097-4547(19961215)46:6<641::AID-JNR1>3.0.CO;2-F.
Effects of thrombin on brain cells, including change of neurite outgrowth and astrocyte shape, are described, but the molecular mechanisms are unclear. We investigated the effects of human alpha-thrombin and a six amino acid thrombin receptor activating peptide (TRAP-6, SFLLRN) on [Ca2+]i, phosphoinositide hydrolysis, and protein kinase C in rat glioma C6 cells. Stimulation of C6 cells with both alpha-thrombin and TRAP-6 resulted in [Ca2+]i mobilization, [3H]Inositol phosphate response, and enhanced immunoreactivity of the protein kinase C (PKC) isoenzymes alpha, beta, gamma, delta, and epsilon. Results suggest that alpha-thrombin and TRAP-6 activate at least partially the same intracellular signaling pathways in rat glioma C6 cells, which is evidence for involvement of "tethered ligand" receptor in thrombin induced signaling in glioma C6 cells.
文中描述了凝血酶对脑细胞的影响，包括神经突生长和星形胶质细胞形态的变化，但分子机制尚不清楚。我们研究了人α-凝血酶和一种六氨基酸凝血酶受体激活肽（TRAP-6，SFLLRN）对大鼠胶质瘤C6细胞中[Ca2+]i、磷酸肌醇水解和蛋白激酶C的影响。用α-凝血酶和TRAP-6刺激C6细胞均导致[Ca2+]i动员、[3H]肌醇磷酸反应，并增强了蛋白激酶C（PKC）同工酶α、β、γ、δ和ε的免疫反应性。结果表明，α-凝血酶和TRAP-6至少部分激活了大鼠胶质瘤C6细胞中相同的细胞内信号通路，这证明“拴系配体”受体参与了胶质瘤C6细胞中凝血酶诱导的信号传导。
