Kaufmann R, Schafberg H, Zieger M, Henklein P, Nowak G
Research Unit, Pharmacological Haemostaseology, Medical Faculty at the Friedrich-Schiller-University Jena, Germany.
Neuropeptides. 1998 Apr;32(2):185-9. doi: 10.1016/s0143-4179(98)90036-1.
Chotecystoknin octapeptide (CCK-8) has been shown to stimulate DNA synthesis in rat glioma C6 cells by activation of CCKB type receptors. However, the signalling pathways contributing to this proliferative action in C6 cells have not been investigated thus far. This study demonstrated that stimulation of rat glioma C6 cells with CCK-8S resulted in activation of protein kinase C isozymes betaI, betaII, gamma and zeta. The participation of protein kinase C in the CCK-8S-induced effect on C6 cell growth was demonstrated by measurement of [3H]thymidine incorporation and estimation of cell number. The data indicate that CCK-8S stimulates growth in rat glioma C6 cells by a protein kinase C-dependent mechanism.
胆囊收缩素八肽（CCK - 8）已被证明可通过激活CCKB型受体来刺激大鼠胶质瘤C6细胞中的DNA合成。然而，迄今为止，尚未研究导致C6细胞这种增殖作用的信号通路。本研究表明，用CCK - 8S刺激大鼠胶质瘤C6细胞会导致蛋白激酶C同工酶βI、βII、γ和ζ的激活。通过测量[3H]胸腺嘧啶核苷掺入量和估计细胞数量，证明了蛋白激酶C参与了CCK - 8S对C6细胞生长的诱导作用。数据表明，CCK - 8S通过蛋白激酶C依赖性机制刺激大鼠胶质瘤C6细胞的生长。
