Sidoni A, Cavaliere A, Alunno P, Bucciarelli E
Institute of Pathologic Anatomy and Histology, University of Perugia, School of Medicine, Italy.
Cytometry. 1996 Dec 15;26(4):293-7. doi: 10.1002/(SICI)1097-0320(19961215)26:4<293::AID-CYTO9>3.0.CO;2-D.
Image cytometric analysis of DNA ploidy status of 40 fresh primary breast carcinomas was performed in a comparative study on cytologic preparations stained directly by the Feulgen method and smears from the same tumors Feulgen-stained after Papanicolaou staining. There was a 0.82 overall concordance in diploid and aneuploid tumor classification and a good correlation (r = 0.736, P < 0.001) between the DNA indices determined by the two methods. Discordances are probably due to tumor heterogeneity. This study demonstrates that image cytometric DNA analysis on previously routine-stained cytologic preparations is feasible and reliable for pretreatment DNA analysis and suggests potential applications in retrospective studies with cytologic material from archives.
对40例新鲜原发性乳腺癌的DNA倍体状态进行了图像细胞分析，这是一项比较研究，研究对象为直接用福尔根法染色的细胞学标本，以及同一肿瘤经巴氏染色后再进行福尔根染色的涂片。在二倍体和非整倍体肿瘤分类方面，总体一致性为0.82，两种方法测定的DNA指数之间具有良好的相关性（r = 0.736，P < 0.001）。不一致可能是由于肿瘤异质性。本研究表明，对先前常规染色的细胞学标本进行图像细胞DNA分析对于预处理DNA分析是可行且可靠的，并提示了其在利用存档细胞学材料进行回顾性研究中的潜在应用。
