Schulte E K, Fink D K
Anatomisches Institut, Johannes Gutenberg-Universität, Mainz, Germany.
Anal Cell Pathol. 1995 Dec;9(4):257-68.
The suitability of commercial and pure aluminum-hematein for quantitative DNA image cytometry was investigated. Cervical smears, breast cancer aspiration biopsies, and rabbit liver tissue imprints were stained with Mayer's and Harris' al-hematein with variable staining times and dye concentrations. Pure and commercial hematoxylin was used. Nucleic acids were removed by enzyme digestion or by HCl-hydrolysis. A standard Feulgen stain served as control. DNA-polyacrylamide films were used as staining models. Absorption was measured using a VIDAS image analyzer. DNA in liver cell nuclei was not stained in a stoichiometric dye-DNA ratio. Sequential staining of cervical smears with hematein followed by the Feulgen reaction gave a covariance between 0.77 and 0.88 for IOD. Photometric errors due to unspecific RNA or protein staining were remarkable. Harris' and Mayer's hematein gave comparable results. Pure hematein gave slightly better results than commercial batches. DNA staining in model films was not quantitative with hematein. Al-hematein should therefore not be used for quantitative DNA cytometry.
研究了商用和纯铝苏木精用于定量DNA图像细胞术的适用性。对宫颈涂片、乳腺癌针吸活检标本和兔肝组织印片，分别用不同染色时间和染料浓度的梅耶氏和哈里斯氏铝苏木精进行染色。使用了纯苏木精和商用苏木精。通过酶消化或盐酸水解去除核酸。以标准福尔根染色作为对照。使用DNA - 聚丙烯酰胺膜作为染色模型。使用VIDAS图像分析仪测量吸光度。肝细胞核中的DNA未以化学计量的染料 - DNA比例染色。宫颈涂片先用苏木精顺序染色，然后进行福尔根反应，积分光密度（IOD）的协方差在0.77至0.88之间。由于非特异性RNA或蛋白质染色导致的光度误差显著。哈里斯氏和梅耶氏苏木精给出了可比的结果。纯苏木精的结果略优于商业批次。在模型膜中，铝苏木精对DNA的染色不是定量的。因此，铝苏木精不应被用于定量DNA细胞术。
