Closse C, Seigneur M, Renard M, Pruvost A, Dumain P, Belloc F, Boisseau M R
Laboratories of Hematology, University of Bordeaux II, Pessac, France.
Haemostasis. 1996 Oct;26 Suppl 4:177-81. doi: 10.1159/000217296.
P-selectin is an endothelial adhesion molecule involved in the initial step of the neutrophil recruitment. We investigated the effect of hypoxia (95% N2, 5% CO2) and of hypoxia-reoxygenation (95% air, 5% CO2) on the expression of P-selectin by human umbilical vein endothelial cells (HUVEC). P-selectin expression was detected by immunolabelling and quantified by flow cytometric analysis. Our data indicate that hypoxia induces an increase in P-selectin expression with a maximum reached after 90 minutes. A hypoxic exposure of 90 minutes results in a highly significant increase compared to normoxia (p < 0.001, n = 13). Furthermore, when a reoxygenation period follows 90 minutes of hypoxia, the initially elevated levels of P-selectin are dramatically enhanced with a maximum obtained after 60 minutes of reoxygenation.
P-选择素是一种内皮黏附分子，参与中性粒细胞募集的起始步骤。我们研究了缺氧（95% N₂，5% CO₂）和缺氧复氧（95%空气，5% CO₂）对人脐静脉内皮细胞（HUVEC）中P-选择素表达的影响。通过免疫标记检测P-选择素表达，并通过流式细胞术分析进行定量。我们的数据表明，缺氧诱导P-选择素表达增加，90分钟后达到最大值。与常氧相比，90分钟的缺氧暴露导致高度显著增加（p < 0.001，n = 13）。此外，当在90分钟缺氧后进行复氧期时，最初升高的P-选择素水平会显著增强，在复氧60分钟后达到最大值。
