Liu Yanyan, Huang Tao, Yap Nicole Alexis, Lim Khoon, Ju Lining Arnold
School of Biomedical Engineering, The University of Sydney, Darlington, NSW, 2008, Australia.
Charles Perkins Centre, The University of Sydney, Camperdown, NSW 2006, Australia.
Bioact Mater. 2024 Sep 5;42:328-344. doi: 10.1016/j.bioactmat.2024.08.040. eCollection 2024 Dec.
Thrombosis, a leading cause of cardiovascular morbidity and mortality, involves the formation of blood clots within blood vessels. Current animal models and systems have limitations in recapitulating the complex human vasculature and hemodynamic conditions, limiting the research in understanding the mechanisms of thrombosis. Bioprinting has emerged as a promising approach to construct biomimetic vascular models that closely mimic the structural and mechanical properties of native blood vessels. This review discusses the key considerations for designing bioprinted vascular conduits for thrombosis studies, including the incorporation of key structural, biochemical and mechanical features, the selection of appropriate biomaterials and cell sources, and the challenges and future directions in the field. The advancements in bioprinting techniques, such as multi-material bioprinting and microfluidic integration, have enabled the development of physiologically relevant models of thrombosis. The future of bioprinted models of thrombosis lies in the integration of patient-specific data, real-time monitoring technologies, and advanced microfluidic platforms, paving the way for personalized medicine and targeted interventions. As the field of bioprinting continues to evolve, these advanced vascular models are expected to play an increasingly important role in unraveling the complexities of thrombosis and improving patient outcomes. The continued advancements in bioprinting technologies and the collaboration between researchers from various disciplines hold great promise for revolutionizing the field of thrombosis research.
血栓形成是心血管疾病发病和死亡的主要原因之一，涉及血管内血凝块的形成。目前的动物模型和系统在重现复杂的人体血管系统和血流动力学条件方面存在局限性，限制了对血栓形成机制的研究。生物打印已成为构建仿生血管模型的一种有前景的方法，该模型能紧密模拟天然血管的结构和力学特性。本文综述了设计用于血栓形成研究的生物打印血管导管的关键考虑因素，包括纳入关键的结构、生化和力学特征，选择合适的生物材料和细胞来源，以及该领域的挑战和未来方向。生物打印技术的进步，如多材料生物打印和微流体集成，已使具有生理相关性的血栓形成模型得以发展。生物打印血栓形成模型的未来在于整合患者特异性数据、实时监测技术和先进的微流体平台，为个性化医疗和靶向干预铺平道路。随着生物打印领域不断发展，这些先进的血管模型有望在揭示血栓形成的复杂性和改善患者预后方面发挥越来越重要的作用。生物打印技术的持续进步以及各学科研究人员之间的合作，为彻底改变血栓形成研究领域带来了巨大希望。
