利用反向PCR技术开发针对易变生丝微菌的物种特异性基因探针。
Development of a species-specific gene probe for Hyphomicrobium facilis with the inverse PCR.
作者信息
Fesefeldt A, Poetsch M, Gliesche C G
机构信息
Institut für Allgemeine Mikrobiologie, Universität Kiel, Germany.
出版信息
Appl Environ Microbiol. 1997 Jan;63(1):335-7. doi: 10.1128/aem.63.1.335-337.1997.
Abstract
A species-specific gene probe for Hyphomicrobium facilis was generated from a transposon Tn5-132 insertion mutant defective in methanol oxidation by the inverse PCR. With this probe, the abundance of H. facilis in a garden soil was determined as a subfraction of the total cultivable hyphomicrobia.
摘要
通过反向PCR从一个在甲醇氧化方面存在缺陷的转座子Tn5-132插入突变体中获得了易变生丝微菌的物种特异性基因探针。利用该探针，将花园土壤中易变生丝微菌的丰度确定为可培养生丝微菌总数的一个亚组分。
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