Ely B
Mol Gen Genet. 1985;200(2):302-4. doi: 10.1007/BF00425440.
We have constructed a series of transposon delivery vectors derived from pRK2013. Since pRK2013 has a broad host range transfer system and a ColE1 replicon, it can be transferred to, but not replicated in, many non-enteric gram-negative bacteria. Thus pRK2013 provides an effective mechanism for the transient introduction of a transposon. Delivery vectors containing Tn7 (tmp str), Tn10 (tet), Tn10 HH104 (tet), or Tn5-132 (tet) have been constructed. When transposition in Caulobacter crescentus was examined, both Tn7 and Tn5-132 were found to transpose efficiently. In contrast, although the antibiotic resistances of Tn10 and Tn501 (mer) were expressed in C. crescentus, no transposition was observed with either transposon. However, transposition of Tn10 from the Tn10 vectors did occur in Acinetobacter calcoaceticus, and transposition of Tn501 from pMD100 has been demonstrated in Rhizobium japonicum (Bullerjahn and Benzinger 1984). Thus, transposon-host interactions play an important role in the determination of whether a particular transposon can transpose in a given host. Furthermore, the results with C. crescentus indicate that there must be different requirements for host interactions for Tn10 and Tn501 than for Tn5 and Tn7.
我们构建了一系列源自pRK2013的转座子递送载体。由于pRK2013具有广泛宿主范围的转移系统和ColE1复制子，它可以转移至许多非肠道革兰氏阴性菌中，但不能在这些菌中复制。因此，pRK2013为转座子的瞬时导入提供了一种有效机制。已构建了含有Tn7（tmp str）、Tn10（tet）、Tn10 HH104（tet）或Tn5 - 132（tet）的递送载体。当检测新月柄杆菌中的转座情况时，发现Tn7和Tn5 - 132都能高效转座。相比之下，虽然Tn10和Tn501（mer）的抗生素抗性在新月柄杆菌中得以表达，但未观察到这两种转座子发生转座。然而，Tn10从Tn10载体发生的转座确实在醋酸钙不动杆菌中出现，并且pMD100中的Tn501在日本根瘤菌中的转座也已得到证实（Bullerjahn和Benzinger，1984）。因此，转座子与宿主的相互作用在决定特定转座子能否在给定宿主中转座方面起着重要作用。此外，新月柄杆菌的实验结果表明，Tn10和Tn501与宿主相互作用的要求必定不同于Tn5和Tn7。
