Matsushita M, Watanabe M, Yamakawa S, Takayama S, Isogai A, Hinata K
Faculty of Agriculture, Tohoku University, Sendai, Japan.
Genes Genet Syst. 1996 Aug;71(4):255-8. doi: 10.1266/ggs.71.255.
We have identified three strains of the same S24-haplotype in self-incompatible B. campestris L. Two of them, S-12j and 1-1j, have been derived from Japanese populations and one, 27-1t, from Turkish one. The cDNA clones of SLG24 (S24-locus glycoprotein), which linked to the S-locus, were isolated from each strain, and sequenced. Each clone isolated from S-12j, 1-1j and 27-1t, was designated as SLG24 (S-12j), SLG24 (1-1j) and SLG24 (27-1t), respectively. Their nucleotide sequences were completely identical in coding region, 5' non-coding region, and 3' non-coding region, though the position of the polyadenylation site was slightly different among the cDNA clones. This result suggests that the origin of S24-haplotype in these three strains might be common, and that the nucleotide sequences of SLG24 of the same S24-haplotype are completely conserved among different populations. The high conservation of the SLG24 nucleotide sequences is probably essential for the recognition of self or non-self of self-incompatibility in this S24-haplotype.
我们在自交不亲和的油菜中鉴定出了三种相同S24单倍型的菌株。其中两种，S-12j和1-1j，源自日本种群，另一种，27-1t，源自土耳其种群。从每个菌株中分离出与S位点连锁的SLG24（S24位点糖蛋白）的cDNA克隆，并进行测序。从S-12j、1-1j和27-1t中分离出的每个克隆，分别命名为SLG24（S-12j）、SLG24（1-1j）和SLG24（27-1t）。它们在编码区、5'非编码区和3'非编码区的核苷酸序列完全相同，尽管在cDNA克隆中多聚腺苷酸化位点的位置略有不同。这一结果表明，这三种菌株中S24单倍型的起源可能是共同的，并且相同S24单倍型的SLG24核苷酸序列在不同种群中是完全保守的。SLG24核苷酸序列的高度保守性可能对于该S24单倍型自交不亲和中自我或非自我的识别至关重要。
