Testet E, Bessoule J J, Mongrand S, Guillot-Salomon T, Cantrel C, Cassagne C
Laboratoire de Biogenèse Membranaire, UMR CNRS 5544, Université Victor Segalen Bordeaux 2, France.
FEBS Lett. 1996 Dec 9;399(1-2):87-91. doi: 10.1016/s0014-5793(96)01293-8.
In the presence of oleoyl-CoA, purified and intact mitochondria from potato tuber formed phosphatidylcholine from labeled lysophosphatidylcholine. The labeled oleoyl moiety of the acyl-CoA was also incorporated in the absence of exogenous lysolipids, such incorporation being largely increased by the addition of exogenous lysophosphatidylcholine. In the presence of various other lysophospholipids, no synthesis of the corresponding phospholipids was observed, suggesting a high specificity of the acyltransferase towards the acyl acceptor. This enzyme was chiefly located in the outer membrane of mitochondria. These results indicate that any acylglycerophosphorylcholine transferred from the endoplasmic reticulum to mitochondria may be acylated to phosphatidylcholine.
在油酰辅酶A存在的情况下，从马铃薯块茎中纯化得到的完整线粒体可利用标记的溶血磷脂酰胆碱合成磷脂酰胆碱。在没有外源溶血脂质的情况下，酰基辅酶A的标记油酰部分也会掺入，而添加外源溶血磷脂酰胆碱会大大增加这种掺入。在存在各种其他溶血磷脂的情况下，未观察到相应磷脂的合成，这表明酰基转移酶对酰基受体具有高度特异性。这种酶主要位于线粒体外膜。这些结果表明，从内质网转移到线粒体的任何酰基甘油磷酸胆碱都可能被酰化形成磷脂酰胆碱。
