Bessoule J J, Testet E, Cassagne C
Laboratoire de Biogenese Membranaire, CNRS-URA 1811, Université de Bordeaux II, France.
Eur J Biochem. 1995 Mar 1;228(2):490-7.
Purified, intact chloroplasts from Allium porrum seedlings are able to synthesize phosphatidylcholine by acylating lysophosphatidylcholine (but not glycerophosphocholine) with acyl-CoAs. The acyltransferase activity is located in the envelope of chloroplasts. It is specific for lysophosphatidylcholine and the neosynthesized lipids have a C18 fatty acid esterified to the sn-2 position of the glycerol backbone ('eukaryotic lipids'). By preincubating endoplasmic reticulum membranes with labeled lysophosphatidylcholine, it was shown that this molecule could be transferred by a partition process from the endoplasmic reticulum to chloroplasts where they are acylated to yield phosphatidylcholine.
从韭葱幼苗中纯化得到的完整叶绿体能够通过用酰基辅酶 A 酰化溶血磷脂酰胆碱（而非甘油磷酸胆碱）来合成磷脂酰胆碱。酰基转移酶活性位于叶绿体包膜中。它对溶血磷脂酰胆碱具有特异性，新合成的脂质在甘油主链的 sn-2 位酯化有一个 C18 脂肪酸（“真核生物脂质”）。通过用标记的溶血磷脂酰胆碱预孵育内质网膜，结果表明该分子可通过分配过程从内质网转移至叶绿体，在叶绿体中它们被酰化生成磷脂酰胆碱。
