Brown J W, Smith P, Simpson C G
Department of Cell and Molecular Genetics, Scottish Crop Research Institute, Dundee, Scotland, UK.
Plant Mol Biol. 1996 Nov;32(3):531-5. doi: 10.1007/BF00019105.
We have analysed 998 Arabidopsis intron sequences in the EMBL database. All Arabidopsis introns to adhere to the :GU ... AG: rule with the exception of 1% of introns with :GC at their 5' ends. Virtually all of the introns contained a putative branchpoint sequence (YUNAN) 18 to 60 nt upstream of the 3' splice site. Although a polypyrimidine tract was much less apparent than in vertebrate introns, the most common nucleotide in the region upstream of the 3' splice site was uridine. Consensus sequences for 5' and 3' splice sites and branchpoint sequences for Arabidopsis introns are presented.
我们分析了EMBL数据库中的998个拟南芥内含子序列。除了1%的内含子在其5'端为:GC外，所有拟南芥内含子均遵循:GU...AG:规则。几乎所有内含子在3'剪接位点上游18至60个核苷酸处都含有一个推定的分支点序列（YUNAN）。尽管多嘧啶序列比脊椎动物内含子中的要明显少得多，但3'剪接位点上游区域最常见的核苷酸是尿苷。本文给出了拟南芥内含子5'和3'剪接位点的共有序列以及分支点序列。
