Simpson C G, Brown J W
Cell and Molecular Genetics Department, Scottish Crop Research Institute, Dundee, UK.
Plant Mol Biol. 1993 Jan;21(2):205-11. doi: 10.1007/BF00019937.
For successful splicing in dicot plants the only recognised intron requirements are 5' and 3' splice sites and AU-rich sequences. We have investigated further the importance of AU-rich elements by analyzing the splicing of an AU-rich antisense intron sequence. Activation of cryptic splice sites on either side of the AU-rich sequence permitted the efficient removal of this essentially non-intron sequence by splicing. This splicing event not only confirms the importance of AU-rich sequences but also has implications for the evolution of interrupted genes and the expression of heterologous genes in transgenic plants.
对于双子叶植物中的成功剪接，唯一公认的内含子要求是5'和3'剪接位点以及富含AU的序列。我们通过分析富含AU的反义内含子序列的剪接，进一步研究了富含AU元件的重要性。富含AU序列两侧的隐蔽剪接位点的激活使得通过剪接能够有效去除这个基本上非内含子的序列。这一剪接事件不仅证实了富含AU序列的重要性，也对间断基因的进化以及转基因植物中异源基因的表达具有启示意义。
