Utsugi S, Sakamoto W, Ogura Y, Murata M, Motoyoshi F
Research Institute for Bioresources, Okayama University, Kurashiki, Japan.
Plant Mol Biol. 1996 Nov;32(4):759-65. doi: 10.1007/BF00020217.
Seventeen cDNA clones of genes corresponding to mRNAs expressed preferentially in floral organs of Arabidopsis thaliana were obtained by differential screening of a flower bud cDNA library, and classified into five groups (1A, 17A, 1B, 4B and 5B) by cross-hybridization and restriction analysis. Sequence analysis revealed that the 1A-1 and 17A-1 clones encode vegetative storage proteins (VSPs). The VSP mRNAs were detected in a small amount in leaves and increased to a limited level by wounding. Both 1B-1 and 5B-1 clones were homologous to transmembrane protein cDNAs. The protein encoded by 4B-1 clone contained a proline-rich region, but no homologous proteins were found in databases.
通过对拟南芥花芽cDNA文库进行差异筛选，获得了17个与在花器官中优先表达的mRNA相对应的基因的cDNA克隆，并通过交叉杂交和限制性分析将其分为五组（1A、17A、1B、4B和5B）。序列分析表明，1A-1和17A-1克隆编码营养贮藏蛋白（VSP）。VSP mRNA在叶片中少量检测到，并通过损伤增加到有限水平。1B-1和5B-1克隆均与跨膜蛋白cDNA同源。4B-1克隆编码的蛋白质含有富含脯氨酸的区域，但在数据库中未发现同源蛋白质。
