Xu L, Zheng L, Coughlan S J, Wang X
Department of Biochemistry, Kansas State University, Manhattan 66506, USA.
Plant Mol Biol. 1996 Nov;32(4):767-71. doi: 10.1007/BF00020218.
Phospholipase D (PLD; EC 3.1.4.4) has been proposed to play a pivotal role in various cellular processes, but molecular understanding of this enzyme is rather limited. This report describes the nucleotide sequence, structure, and genomic organization of a PLD gene from castor bean (Ricinus communis L. cv. Hale). The PLD gene was isolated from a castor bean genomic library using the PLD cDNA as a hybridization probe. Sequence comparison with the PLD cDNA revealed that the PLD gene consisted of four exons and three introns, one of which interrupts the 5'-untranslated region. Southern blot analysis indicated that the cloned PLD gene was present as a single-copy gene, and yet there were other PLD or PLD-related sequences in the castor bean genome.
磷脂酶D（PLD；EC 3.1.4.4）被认为在各种细胞过程中起关键作用，但对这种酶的分子理解相当有限。本报告描述了蓖麻（Ricinus communis L. cv. Hale）中一个PLD基因的核苷酸序列、结构和基因组组织。使用PLD cDNA作为杂交探针从蓖麻基因组文库中分离出PLD基因。与PLD cDNA的序列比较表明，PLD基因由四个外显子和三个内含子组成，其中一个内含子打断了5'-非翻译区。Southern印迹分析表明，克隆的PLD基因以单拷贝基因形式存在，但蓖麻基因组中还有其他PLD或PLD相关序列。
