用于快速诊断雏鸡大规模坏死性禽腺病毒肝炎和胰腺炎的DNA原位杂交技术
DNA in situ hybridization for the rapid diagnosis of massive necrotizing avian adenovirus hepatitis and pancreatitis in chicks.
作者信息
Goodwin M A, Latimer K S, Resurreccion R S, Miller P G, Campagnoli R P
机构信息
Georgia Poultry Laboratory, Oakwood 30566, USA.
出版信息
Avian Dis. 1996 Oct-Dec;40(4):828-31.
PMID:8980813
Abstract
The present study describes the use of DNA in situ hybridization for the rapid diagnosis of massive necrotizing adenovirus hepatitis and pancreatitis in broiler chicks. A light microscope and DNA probes were used to identify avian adenovirus in replicate sections of formalin-fixed paraffin-embedded liver and pancreas from field and experimental chicks. Avian adenovirus infection was confirmed by transmission electron microscopy and virus isolation.
摘要
本研究描述了利用DNA原位杂交技术快速诊断肉鸡雏鸡的大规模坏死性腺病毒肝炎和胰腺炎。使用光学显微镜和DNA探针，在来自田间和实验雏鸡的福尔马林固定石蜡包埋肝脏和胰腺的重复切片中鉴定禽腺病毒。通过透射电子显微镜和病毒分离确认了禽腺病毒感染。
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