Tullio V, Cuffini A M, De Leo C, Perrone F, Carlone N A
Department of Public Health and Microbiology, University of Turin, Italy.
J Chemother. 1996 Dec;8(6):438-44. doi: 10.1179/joc.1996.8.6.438.
In recent years, medical interest in evaluating the interaction among mycetes, phagocytes, and antimycotic drugs has increased notably due to higher incidences of fungal infections in immunocompromised subjects and to the long-term therapy they require. In this study the in vitro and ex vivo interaction of fluconazole, at plasma concentrations, with mouse macrophages was evaluated in the presence of different inocula of Candida albicans. The results showed that fluconazole did not interfere negatively with phagocyte functions; conversely, according to different experimental conditions, it was able to increase both phagocytosis and intracellular killing of candida, probably exerting its action on the yeast rather than on the phagocyte. A higher enhancement of macrophage functions was observed in vitro when the drug was present in the medium with macrophages and candida in a 1:1 ratio.
近年来，由于免疫功能低下患者真菌感染的发生率较高以及他们所需的长期治疗，医学上对评估真菌、吞噬细胞和抗真菌药物之间相互作用的兴趣显著增加。在本研究中，在存在不同接种量白色念珠菌的情况下，评估了血浆浓度的氟康唑与小鼠巨噬细胞的体外和离体相互作用。结果表明，氟康唑不会对吞噬细胞功能产生负面影响；相反，根据不同的实验条件，它能够增加对念珠菌的吞噬作用和细胞内杀伤作用，可能是对酵母发挥作用而非对吞噬细胞。当药物以1:1的比例存在于含有巨噬细胞和念珠菌的培养基中时，在体外观察到巨噬细胞功能有更高的增强。
