Tsuge H, Maeno M, Hayakawa T, Suzuki Y
Department of Food Science, Faculty of Agriculture, Gifu University, Japan.
J Nutr Sci Vitaminol (Tokyo). 1996 Oct;42(5):377-86. doi: 10.3177/jnsv.42.377.
The nutritional effects of pyridoxine-alpha-glucoside (PN-alpha-Glc), pyridoxine-5' beta-glucoside (PN-5' beta-Glc) and epsilon-N-(phosphopyridoxyl)-lysine (PNP-Lys) were examined by means of: 1) transport across the intestinal wall using everted rat intestine, 2) metabolic conversion by liver or kidney homogenate, and 3) oral administration of the compound to B6-deficient rats and the subsequent analysis of B6 derivatives found in plasma. Using everted sacs prepared from rat small intestine, PN-alpha-Glc was transported into the serosal side in its intact form. On the other hand, a part of PN-5' beta-Glc was found as PN on the serosal side (PN-5' beta-Glc: PN = 2:1). When PN-alpha-Glc, PN-5' beta-Glc or PNP-Lys was incubated with liver or kidney homogenate for 3 h at pH 6.0, PN-alpha-Glc was hydrolyzed to PN (6%), while there was no hydrolysis of PN-5' beta-Glc. After 30 min of administration of each B6 derivative to B6-deficient rats, blood was collected from the heart, and the B6-derivatives found in plasma were analyzed. It was ascertained that PN-alpha-Glc served as well as PN as a B6 nutrient, while PN-5' beta-Glc and PNP-Lys were not easily metabolized to the coenzyme form, pyridoxal 5'-phosphate.
通过以下方式研究了吡哆醇 -α-葡萄糖苷（PN-α-Glc）、吡哆醇 -5'-β-葡萄糖苷（PN-5'-β-Glc）和ε-N-（磷酸吡哆醛基）-赖氨酸（PNP-Lys）的营养作用：1）使用外翻大鼠肠进行跨肠壁转运；2）通过肝脏或肾脏匀浆进行代谢转化；3）给维生素B6缺乏的大鼠口服该化合物，随后分析血浆中发现的维生素B6衍生物。使用大鼠小肠制备的外翻囊，PN-α-Glc以完整形式转运到浆膜侧。另一方面，在浆膜侧发现一部分PN-5'-β-Glc以PN形式存在（PN-5'-β-Glc：PN = 2：1）。当PN-α-Glc、PN-5'-β-Glc或PNP-Lys在pH 6.0下与肝脏或肾脏匀浆孵育3小时时，PN-α-Glc水解为PN（6%），而PN-5'-β-Glc未发生水解。给维生素B6缺乏的大鼠分别给予每种维生素B6衍生物30分钟后，从心脏采血，并分析血浆中发现的维生素B6衍生物。已确定PN-α-Glc作为维生素B6营养素的作用与PN相同，而PN-5'-β-Glc和PNP-Lys不易代谢为辅酶形式的磷酸吡哆醛。
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