Maeno M, Morimoto Y, Hayakawa T, Suzuki Y, Tsuge H
Department of Food Science, Faculty of Agriculture, Gifu University, Japan.
Int J Vitam Nutr Res. 1997;67(6):444-9.
In order to compare the nutritional effect of vitamin B6 derivatives, long-term feeding experiments with rats were carried out using pyridoxine-alpha-D-glucoside (PN-alpha-Glc), pyridoxine-beta-D-glucoside (PN-beta-Glc) or epsilon- (N-phosphopyridoxyl)lysine (PNP-Lys) with test diets consisting of basically the AIN-76 composition, except for the addition of 0.1 mg pyridoxine equivalent (PN eq.)/100 g diet. During 21 days of pair-feeding against the vitamin B6-deficient diet group, body weight gain, urinary excretion of xanthurenic acid and pyridoxic acid were measured. After the feeding experiment, rats were killed and examined in terms of liver kynureninase activity (EC 3.7.1.3) with and without adding exogenous pyridoxal 5'-phosphate (PLP), erythrocyte aspartate aminotransferase activity (EC 2.6.1.1), as well as PLP concentration in blood. Rats fed with PN-alpha-Glc grew well, relative to the PN group. On the contrary, PN-beta-Glc poorly served as vitamin B6 source, because average bioavailability was only about 22% in comparison to that of PN (100%). From this long-term feeding experiments, we have shown that PN-alpha-Glc (average bioavailability about 84%) is a good source of vitamin B6 similar to PN.
为了比较维生素B6衍生物的营养效果，以除添加0.1毫克维生素B6当量（PN当量）/100克饲料外基本为AIN - 76组成的试验饲料，用吡哆醇-α-D-葡萄糖苷（PN-α-Glc）、吡哆醇-β-D-葡萄糖苷（PN-β-Glc）或ε-（N-磷酸吡哆醛基）赖氨酸（PNP-Lys）对大鼠进行长期喂养实验。在与维生素B6缺乏饲料组进行21天的配对喂养期间，测量体重增加、尿中黄尿酸和吡哆酸的排泄量。喂养实验结束后，处死大鼠，检测添加和不添加外源磷酸吡哆醛（PLP）时肝脏犬尿氨酸酶活性（EC 3.7.1.3）、红细胞天冬氨酸转氨酶活性（EC 2.6.1.1）以及血液中PLP浓度。相对于PN组，用PN-α-Glc喂养的大鼠生长良好。相反，PN-β-Glc作为维生素B6来源的效果不佳，因为与PN（100%）相比，其平均生物利用率仅约为22%。通过这次长期喂养实验，我们已表明PN-α-Glc（平均生物利用率约84%）是与PN类似的良好维生素B6来源。
