Barber J S, Payne-Johnson C E, Trees A J
Department of Parasitology, Liverpool School of Tropical Medicine, University of Liverpool.
J Small Anim Pract. 1996 Dec;37(12):568-74. doi: 10.1111/j.1748-5827.1996.tb02332.x.
Six cases of neosporosis in dogs were diagnosed on the basis of clinical signs, serology and immunohistochemistry. The brains and spinal cords, at several levels, and various other tissues were examined using anti-Neospora caninum serum by an immunoperoxidase technique. Parasite density varied markedly from case to case. Although found most consistently in the cerebrum, parasites were distributed throughout the central nervous system (CNS), both within the grey and white matter and within nerve roots. Clinical signs were not related to the position of parasites. Tissue cysts were found infrequently in all areas of the CNS, but not in other tissues. Parasite density was not related to the age of the dog or whether treatment had been given. However, with the exception of the only adult dog examined, more CNS parasites were found in dogs with a longer duration of illness and with higher antibody titres. Tachyzoites were present in skeletal muscles, in the muscularis of the oesophagus, in heart, lung and, less frequently, liver, and rarely in the adrenal gland, thyroid gland and uterus; no clinical signs were seen resulting from damage to these organs. Parasites were not observed in lymphoid tissue. In visceral organs, parasites were most widely distributed in peracute cases. For post mortem diagnosis of neosporosis, the CNS, particularly the cerebrum, is the optimum tissue to examine but parasites may also be found in many other tissues, especially in acute cases. Muscle biopsy of appropriate muscles (as suggested by the clinical signs) provides the possibility of a definitive premortem diagnosis.
根据临床症状、血清学和免疫组织化学诊断出6例犬新孢子虫病。采用免疫过氧化物酶技术，使用抗犬新孢子虫血清对大脑和脊髓的多个层面以及其他各种组织进行了检查。不同病例之间的寄生虫密度差异显著。尽管在大脑中发现寄生虫的情况最为一致，但寄生虫分布于整个中枢神经系统（CNS），包括灰质和白质以及神经根内。临床症状与寄生虫的位置无关。在中枢神经系统的所有区域中，组织囊肿很少见，但在其他组织中未发现。寄生虫密度与犬的年龄或是否接受过治疗无关。然而，除了唯一检查的成年犬外，病程较长且抗体滴度较高的犬中枢神经系统中发现的寄生虫更多。速殖子存在于骨骼肌、食管肌层、心脏、肺中，较少见于肝脏，极少见于肾上腺、甲状腺和子宫；这些器官受损未出现临床症状。在淋巴组织中未观察到寄生虫。在内脏器官中，寄生虫在超急性病例中分布最广。对于新孢子虫病的死后诊断，中枢神经系统，尤其是大脑，是最佳的检查组织，但在许多其他组织中也可能发现寄生虫，尤其是在急性病例中。对适当肌肉进行肌肉活检（根据临床症状提示）提供了生前确诊的可能性。
