Klimaschewski L, Hauser C, Heym C
Institute of Anatomy and Cell Biology, University of Heidelberg, Germany.
Neuroreport. 1996 Nov 4;7(15-17):2797-801. doi: 10.1097/00001756-199611040-00078.
The presence and regulation of pituitary adenylate cyclase activating peptide (PACAP) was investigated in the adult rat superior cervical ganglion. Double-labelling immunofluorescence using specific antibodies against PACAP and vasoactive intestinal peptide (VIP) revealed a dense innervation of postganglionic sympathetic neurones by PACAP-immunoreactive but VIP-negative nerve endings that mainly were of preganglionic origin since they disappeared after transection of the cervical sympathetic trunk. Since PACAP regulates transmitter synthesis and release in sympathetic neurones in vitro, this finding provides a morphological correlate for modulatory effects of PACAP in vivo. Few neurones contained PACAP under normal conditions. However, the number of PACAP-positive neurones increased in response to axotomy of the postganglionic nerves. Many of the newly PACAP-positive neurones also contained VIP, suggesting that both peptides are regulated by similar mechanisms.
在成年大鼠颈上神经节中研究了垂体腺苷酸环化酶激活肽（PACAP）的存在及其调节机制。使用抗PACAP和血管活性肠肽（VIP）的特异性抗体进行双重标记免疫荧光显示，PACAP免疫反应阳性但VIP阴性的神经末梢对节后交感神经元有密集的神经支配，这些神经末梢主要起源于节前，因为在切断颈交感干后它们消失了。由于PACAP在体外调节交感神经元中递质的合成和释放，这一发现为PACAP在体内的调节作用提供了形态学上的关联。在正常情况下，很少有神经元含有PACAP。然而，节后神经切断后，PACAP阳性神经元的数量增加。许多新的PACAP阳性神经元也含有VIP，这表明这两种肽受相似机制的调节。
