Nathan P, Sollmann P
Tissue Antigens. 1977 Aug;10(2):65-9. doi: 10.1111/j.1399-0039.1977.tb01120.x.
A two-stage procedure is described for cytotoxicity tests in which three different sera from a prospective kidney recipient are pooled in each well of a standard microcytotoxicity test plate. The results are reproducible and as sensitive as those obtained with single sera tests. The technique permits a reduction in time required for a test and the number of wells that must be evaluated to screen a series of sera. It enables a laboratory to run all available sera on patients for cadaver kidney crossmatching and to eliminate hyperacute rejections. Serum pooling may also be used for screening of many sera against random cell panels.
本文描述了一种用于细胞毒性测试的两阶段程序，即在标准微细胞毒性测试板的每个孔中汇集来自一名潜在肾移植受者的三种不同血清。结果具有可重复性，并且与单血清测试获得的结果一样灵敏。该技术可以减少测试所需的时间以及为筛选一系列血清而必须评估的孔数。它使实验室能够对患者的所有可用血清进行尸体肾交叉配型，并消除超急性排斥反应。血清汇集也可用于针对随机细胞板筛选多种血清。
