Houk E J
Tissue Cell. 1977;9(1):103-18. doi: 10.1016/0040-8166(77)90052-0.
The midgut ultrastructure of rasin-and blood-fed female mosquitoes, Culex tarsalis Coquillentt, was examined. The raisin-fed midgut is characterized by: (1) large nuclei, (2) small mitochondria, (3) short segments of rough endoplasmic reticulum, (4) rough endoplasmic reticular vesicular vesicles in the posterior midgut only, and (5) increased autophagic acitivity with age. Blood feeding elicits drastic changes in midgut epithelial structures: (1) nuclei are smaller, (2) mitochondria are much enlarged, (3) rough endoplasmic reticular vesicles disappear, (4) rough endoplasmic reticular whorls appear, (5) residual lyosomal figures are abundant, and (6) an intercellular accumulation of an electron-opaque material is noted. The significance of rough endoplasmic reticular whorls and vesicles in bloodmeal digestion is discussed. In addition, the concept of a functional host 'gut barrier' to infection by pathogens is examined as related to a possible by-pass mechanism.
对以葡萄干和血液为食的雌性塔氏库蚊（Culex tarsalis Coquillentt）的中肠超微结构进行了检查。以葡萄干为食的中肠具有以下特征：（1）细胞核大；（2）线粒体小；（3）粗面内质网片段短；（4）仅在中肠后部有粗面内质网囊泡；（5）自噬活性随年龄增加。吸食血液会引起中肠上皮结构的剧烈变化：（1）细胞核变小；（2）线粒体大大增大；（3）粗面内质网囊泡消失；（4）出现粗面内质网漩涡；（5）残余溶酶体形态丰富；（6）观察到细胞间有电子不透明物质积累。讨论了粗面内质网漩涡和囊泡在血餐消化中的意义。此外，还研究了功能性宿主“肠道屏障”对病原体感染的概念以及与之相关的可能的旁路机制。
