细菌趋化作用中的磷酸化和去磷酸化蛋白复合物
Phosphorylating and dephosphorylating protein complexes in bacterial chemotaxis.
作者信息
Wang H, Matsumura P
机构信息
Department of Microbiology and Immunology, University of Illinois at Chicago, 60612-7344, USA.
出版信息
J Bacteriol. 1997 Jan;179(1):287-9. doi: 10.1128/jb.179.1.287-289.1997.
Abstract
During optimal motility conditions, a 1:1 stoichiometry of CheA(L) (654 amino acids) to CheA(S) (557 amino acids) was determined. It was also found that CheZ binding to CheA(S) was inhibited by CheA(L)-CheA(S)-CheW interaction. This suggests that CheA(S) has different functions in the phosphorylating complex (CheA(L)-CheA(S)-CheW) and in the dephosphorylating complex (CheA(S)-CheZ).
摘要
在最佳运动条件下，确定了CheA(L)（654个氨基酸）与CheA(S)（557个氨基酸）的化学计量比为1:1。还发现CheZ与CheA(S)的结合受到CheA(L)-CheA(S)-CheW相互作用的抑制。这表明CheA(S)在磷酸化复合物（CheA(L)-CheA(S)-CheW）和去磷酸化复合物（CheA(S)-CheZ）中具有不同的功能。
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本文引用的文献
1
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Mol Microbiol. 1996 Feb;19(4):695-703. doi: 10.1046/j.1365-2958.1996.393934.x.
2
Multiple factors underlying the maximum motility of Escherichia coli as cultures enter post-exponential growth.
J Bacteriol. 1993 Oct;175(19):6238-44. doi: 10.1128/jb.175.19.6238-6244.1993.
3
Assembly and function of a quaternary signal transduction complex monitored by surface plasmon resonance.
Nature. 1993 Sep 23;365(6444):343-7. doi: 10.1038/365343a0.
4
Kinetics of CheA autophosphorylation and dephosphorylation reactions.
Biochemistry. 1994 Jun 28;33(25):7917-24. doi: 10.1021/bi00191a019.
5
The smaller of two overlapping cheA gene products is not essential for chemotaxis in Escherichia coli.
J Bacteriol. 1995 May;177(10):2713-20. doi: 10.1128/jb.177.10.2713-2720.1995.
6
Phosphorylation of three proteins in the signaling pathway of bacterial chemotaxis.
Cell. 1988 Apr 8;53(1):79-87. doi: 10.1016/0092-8674(88)90489-8.
7
Histidine phosphorylation and phosphoryl group transfer in bacterial chemotaxis.
Nature. 1988 Nov 10;336(6195):139-43. doi: 10.1038/336139a0.
8
Bacterial chemotaxis signaling complexes: formation of a CheA/CheW complex enhances autophosphorylation and affinity for CheY.
Proc Natl Acad Sci U S A. 1991 Jul 15;88(14):6269-73. doi: 10.1073/pnas.88.14.6269.
9
Signal transduction in bacteria: CheW forms a reversible complex with the protein kinase CheA.
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10
Signal transduction pathways involving protein phosphorylation in prokaryotes.
Annu Rev Biochem. 1991;60:401-41. doi: 10.1146/annurev.bi.60.070191.002153.
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