Hishida T, Iwasaki H, Ishioka K, Shinagawa H
Department of Molecular Microbiology, Japan.
Gene. 1996 Dec 5;182(1-2):63-70. doi: 10.1016/s0378-1119(96)00474-x.
In Escherichia coli, the products of the ruvA, ruvB and ruvC genes are all involved in the processing of recombination intermediates (Holliday structures) into recombinant molecules. We cloned a 9.4-kb DNA fragment from Pscudomonas aeruginosa PAO1 in a plasmid by functional complementation of the UV sensitivity of an E. coli strain with ruvABC deleted. In P. aeruginosa, the ruv region seemed to form a non-SOS regulated single operon consisting of orf26-ruvC-ruvA-ruvB, while in this region of E. coli, ruvA and ruvB form an SOS-regulated operon, orf26 and ruvC form a non-SOS operon, and these two operons are split by orf23. The deduced amino acid sequences of P. aeruginosa RuvA, RuvB and RuvC proteins were 55, 72 and 55% identical to those of the corresponding E. coli Ruv proteins. The individual ruv genes of P. aeruginosa complemented the corresponding single ruv mutations of E. coli, suggesting that the P. aeruginosa Ruv proteins can interact functionally with their E. coli Ruv partners in forming heterologous complexes. The sequence alignments of the Ruv proteins were extended by incorporation of data about the putative ruv genes obtained from data banks, and the RuvB sequences were conspicuously more conserved than the RuvA and RuvC sequences.
在大肠杆菌中，ruvA、ruvB和ruvC基因的产物均参与将重组中间体（霍利迪结构）加工成重组分子的过程。我们通过功能互补，利用缺失ruvABC的大肠杆菌菌株的紫外线敏感性，从铜绿假单胞菌PAO1中克隆了一个9.4 kb的DNA片段到质粒中。在铜绿假单胞菌中，ruv区域似乎形成了一个由orf26 - ruvC - ruvA - ruvB组成的非SOS调控的单一操纵子，而在大肠杆菌的该区域，ruvA和ruvB形成一个SOS调控的操纵子，orf26和ruvC形成一个非SOS操纵子，并且这两个操纵子被orf23隔开。铜绿假单胞菌RuvA、RuvB和RuvC蛋白推导的氨基酸序列与相应的大肠杆菌Ruv蛋白的序列分别有55%、72%和55%的同源性。铜绿假单胞菌的各个ruv基因可互补大肠杆菌相应的单个ruv突变，这表明铜绿假单胞菌的Ruv蛋白在形成异源复合物时能与其大肠杆菌的Ruv伙伴在功能上相互作用。通过纳入从数据库获得的关于假定ruv基因的数据，扩展了Ruv蛋白的序列比对，并且RuvB序列明显比RuvA和RuvC序列更保守。
